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KMID : 1007520020110020122
Food Science and Biotechnology
2002 Volume.11 No. 2 p.122 ~ p.126
Characteristics of FIA-type Hypoxanthine Sensors Prepared by Some Immobilization Methods
Park, In Seon
Kim, Nam Soo/Noh, Bong Soo/Choi, Boo Dol
Abstract
Xanthine oxidase was immobilized onto various inorganic and organic supports such as Immobilon cellulose nitrate membrane, nylon net, and aminopropylsilylated CPG and Chitopearl 3001 beads by physical adsorption, covalent binding, and ionic and hydrophobic binding. The hypoxanthine sensors thus prepared showed characteristic features regarding temperature and pH profile, and linearity range. The sensors also exhibited improved long-term stability, compared to the free enzyme.
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